Antibody was affinity purified using an epitope specific to Chk1 immobilized on solid support. The epitope recognized by A300-161A maps to a region between residues 200 and 250 of human Checkpoint Kinase 1 using the numbering given in entry NP_001265.1 (GeneID 1111). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
cell cycle checkpoint kinase, Checkpoint kinase-1, Checkpoint, S. pombe, homolog of, 1, CHK1, CHK1 checkpoint homolog, Chk1-S, serine/threonine-protein kinase Chk1
between 200 and 250
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human Chk1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody A300-161A (lot A300-161A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.