Goat anti-Chk1 Antibody, Affinity Purified - A300-161A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to Chk1 immobilized on solid support. The epitope recognized by A300-161A maps to a region between residues 200 and 250 of human Checkpoint Kinase 1 using the numbering given in entry NP_001265.1 (GeneID 1111). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP
Detection of human Chk1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody A300-161A (lot A300-161A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human Chk1 by western blot. Samples: Whole cell lysate (30 µg) from HeLa cells treated with 10 Gy ionizing radiation (IR) or untreated. Antibody: Affinity purified goat anti-Chk1 (Cat. No. A300-161A) used at 0.1 µg/ml. Detection: Chemiluminescence with 30 second exposure.
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