Antibody was affinity purified using an epitope specific to ATM immobilized on solid support. The epitope recognized by A300-135A maps to a region between residues 1750 and 1800 of human Ataxia Telangiectasia Mutated using the numbering given in entry NP_000042.2 (GeneID 472). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
AT mutated, A-T mutated, AT1, ATA, ataxia telangiectasia mutated, ATC, ATD, ATDC, ATE, serine-protein kinase ATM, TEL1, TEL1, telomere maintenance 1, homolog, TELO1
between 1750 and 1800
2 - 8°C
Detection of human ATM by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATM antibody A300-135A (lot A300-135A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Detection of human ATM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATM antibody A300-135A (lot A300-135A-2) used for IP at 3 µg per reaction. ATM was also immunoprecipitated by rabbit anti-ATM antibody BL116. For blotting immunoprecipitated ATM, A300-135A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.