Antibody was affinity purified using an epitope from human BLM immobilized on solid support. The epitope recognized by A300-120A maps to a region between residues 100 and 150 of human Bloom Syndrome using the numbering given in entry NP_000048.1 (GeneID 641). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Bloom syndrome protein, Bloom syndrome RecQ like helicase, Bloom syndrome, RecQ helicase-like, BS, DNA helicase, RecQ-like type 2, MGRISCE1, recQ protein-like 3, RECQ2, RECQL2, RECQL3
between 100 and 150
2 - 8°C
Detection of human BLM by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody A300-120A (lot A300-120A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human BLM by western blot and immunoprecipitation. Sample: Whole cell lysate from HEK293T cells (35 µg for WB; 775 µg for IP). Antibody: Affinity purified goat anti-BLM A300-120A used at 0.5 µg/ml for WB and 3 µg/mg lysate for IP. immunoprecipitatesd BLM was detected by western blot using affinity purified rabbit anti-BLM A300-110A. Detection: Chemiluminescence.