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Rabbit anti-RbBP5 Antibody, Affinity Purified

Catalogue number:
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00 Estimated delivery Wed 27 Mar
Shipping is calculated in checkout
Applications:

WB, IP, IHC, ICC, ChIP, ChIP-chip, ChIP-Seq

Antibody Host:

Rabbit

Target:

RbBP5

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

between 500 and C-term

Alternative Names:

SWD1; RBQ3; SWD1, Set1c WD40 repeat protein, homolog; retinoblastoma-binding protein 5; RBBP-5; retinoblastoma-binding protein RBQ-3

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Product Description:

Rabbit anti-RbBP5 Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human RbBP5 by western blot
Detection of human RbBP5 by western blot. Sample: Whole cell lysate (50 µg for WB and Input; one 10 cm plate for IP) from HEK293T cells. Antibody: Affinity purified rabbit anti-RbBP5 antibody A300-109A used at the indicated concentrations for WB or 10 µg for IP (15% of IP loaded). immunoprecipitatesd RbBP5 was detected by WB using 0.2 mg/ml. Detection: Chemiluminescence with 1 second (WB) or 10 second (IP/WB) exposure.
ChIP-chip scatter plot of anti-RbBP5 (A3
ChIP-chip scatter plot of anti-RbBP5 (A300-109A) enriched DNA binding sites versus input reference DNA. A. 10 µg of A300-109A was used to immunoprecipitate chromatin from K-562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). immunoprecipitatesd DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dNTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-RbBP5 ChIP, normal rabbit IgG showed little enrichment.
Detection of human RbBP5 by immunohistoc
Detection of human RbBP5 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- RbBP5 (Cat. No. A300-109A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
Localization of RbBP5 Binding Sites by C
Localization of RbBP5 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-RbBP5 antibody A300-109A and analyzed by DNA sequencing. The figure illustrates the peak distribution of RbBP5 binding within a 500 Kb region of chromosome 1 as detected using anti-RbBP5 antibody A300-109A. ChIP-seq validation performed by Diogenode, Denville, NJ.
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