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Rabbit anti-RbBP5 Antibody Affinity Purified

Catalogue number:
Size:
100 µl (1000 µg/ml)
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£386.40 Estimated delivery Fri 10 Apr
Special price until 01 Dec 2026
Normal price £483.00
Shipping is calculated in checkout
Applications:

ChIP,ICC,IHC,IP,WB

Antibody Host:

Rabbit

Target:

RbBP5

Species Reactivity:

Human;Mouse

Immunogen:

Between 488 and C-terminus

Alternative Names:

RBBP-5; RBQ3; retinoblastoma-binding protein 5; retinoblastoma-binding protein RBQ-3; SWD1; SWD1, Set1c WD40 repeat protein, homolog

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Product Description:

Rabbit anti-RbBP5 Antibody Affinity Purified - 100 µl (1000 µg/ml)

Detection of human RbBP5 by western blot
Detection of human RbBP5 by western blot. Sample: Whole cell lysate (50 µg for WB and Input; one 10 cm plate for IP) from HEK293T cells. Antibody: Affinity purified rabbit anti-RbBP5 antibody A300-109A used at the indicated concentrations for WB or 10 µg for IP (15% of IP loaded). immunoprecipitatesd RbBP5 was detected by WB using 0.2 mg/ml. Detection: Chemiluminescence with 1 second (WB) or 10 second (IP/WB) exposure.
ChIP-chip scatter plot of anti-RbBP5 (A3
ChIP-chip scatter plot of anti-RbBP5 (A300-109A) enriched DNA binding sites versus input reference DNA. A. 10 µg of A300-109A was used to immunoprecipitate chromatin from K-562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). immunoprecipitatesd DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dNTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-RbBP5 ChIP, normal rabbit IgG showed little enrichment.