Antibody was affinity purified using an epitope specific to Menin immobilized on solid support. The epitope recognized by A300-106A maps to a region between residue 575 and the C-terminus (residue 615) of human Multiple Endocrine Neoplasia 1 using the numbering given in entry NP_000235.2 (GeneID 4221). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
MEAI, menin, SCG2
between 575 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human Menin by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Menin antibody A300-106A (lot A300-106A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human Menin by western blot and immunoprecipitation. Samples: Whole cell lysate (40 µg for WB; one 10 cm plate HEK293T cells for IP) from 293T cells. Antibodies: Affinity purified goat anti-Menin A300-106A used as indicated for IP. Menin was detected by western blot using affinity purified rabbit anti-Menin A300-105A at 0.1 µg/ml for WB. Detection: Chemiluminescence exposed for 1 minute.