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Goat anti-Menin Antibody, Affinity Purified

Catalogue number:
A300-106A
Size:
100 µl
Product is available in:
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Antibody was affinity purified using an epitope specific to Menin immobilized on solid support. The epitope recognized by A300-106A maps to a region between residue 575 and the C-terminus (residue 615) of human Multiple Endocrine Neoplasia 1 using the numbering given in entry NP_000235.2 (GeneID 4221). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Product Type:

Antibodies

Alternative Names:

MEAI, menin, SCG2

Species Reactivity:

Human

Immunogen:

between 575 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Goat

Antibody Isotype:

Whole IgG

Formulation:

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Menin by western blot

Detection of human Menin by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Menin antibody A300-106A (lot A300-106A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Menin by western blot

Detection of human Menin by western blot and immunoprecipitation. Samples: Whole cell lysate (40 µg for WB; one 10 cm plate HEK293T cells for IP) from 293T cells. Antibodies: Affinity purified goat anti-Menin A300-106A used as indicated for IP. Menin was detected by western blot using affinity purified rabbit anti-Menin A300-105A at 0.1 µg/ml for WB. Detection: Chemiluminescence exposed for 1 minute.