Antibody was affinity purified using an epitope specific to SCC-112 immobilized on solid support. The epitope recognized by A300-089A maps to a region between residue 1250 and the C-terminus (residue 1297) of human SCC-112 using the number given in entry NP_056015.1 (GeneID 23244). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
cell proliferation-inducing gene 54 protein, PDS5, regulator of cohesion maintenance, homolog A, PIG54, SCC112, SCC-112, sister chromatid cohesion protein 112, sister chromatid cohesion protein PDS5 homolog A
between 1250 and C-term
2 - 8°C
Detection of human SCC-112 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SCC-112 antibody A300-089A (lot A300-089A-1) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human SCC-112 by western blot and immunoprecipitation. Samples: Nuclear extract (5 and 20 µg for WB, 1 mg for IP) from HeLa cells. Antibodies: Affinity purified rabbit anti-SCC-112 antibody A300-089A used at 0.1 µg/ml for WB and at 2 µg/mg extract for IP. immunoprecipitatesd SCC-112 was detected with affinity purified rabbit anti-SCC-112 antibody A300-088A used at 0.06 µg/ml. Detection: Chemiluminescence with an exposure time of less than 5 minutes.