Antibody was affinity purified using an epitope specific to MLL1 immobilized on solid support. The epitope recognized by A300-087A maps to a region between residues 1320 and 1380 of human myeloid/lymphoid or mixed-lineage leukemia 1 using the number given in Swiss-Prot entry Q03164 (GeneID 4297). The epitope is found in the N-terminal 300 kDa fragment generated by proteolytic cleavage. The epitope is not found in isoform 14P-18B of MLL1. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ALL-1, CXXC7, CXXC-type zinc finger protein 7, histone-lysine N-methyltransferase 2A, HRX, HTRX1, lysine (K)-specific methyltransferase 2A, lysine N-methyltransferase 2A, mixed lineage leukemia 1, MLL, MLL1, MLL1A, Myeloid/lymphoid or mixed-lineage leukem
between 1320 and 1380
2 - 8°C
Detection of human MLL1 by western blot. Samples: Nuclear Extract (15 and 50 µg) from HeLa, HEK293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-MLL1 antibody A300-087A (lot A300-087A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human MLL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MLL1 antibody A300-087A (lot A300-087A-3) used for IP at 6 µg per reaction. MLL1 was also immunoprecipitated by rabbit anti-MLL1 recombinant monoclonal antibody [BL-175-7E8] (A700-010) and rabbit anti-MLL1 antibody A300-086A. For blotting immunoprecipitated MLL1, A700-010 was used at 1:1000. Chemiluminescence with an exposure time of 3 minutes.