Antibody was affinity purified using an epitope specific to Rad21 immobilized on solid support. The epitope recognized by A300-080A maps to a region between residue 575 and the C-terminus (residue 631) human Rad21 homolog using the numbering given in entry NP_006256.1 (GeneID 5885). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CDLS4, double-strand-break repair protein rad21 homolog, hHR21, HR21, HRAD21, kleisin, MCD1, MGS, nuclear matrix protein 1, NXP1, NXP-1, protein involved in DNA double-strand break repair, RAD21 homolog, SCC1, SCC1 homolog, sister chromatid cohesion 1
between 575 and C-term
2 - 8°C
Detection of human and mouse Rad21 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad21 antibody A300-080A (lot A300-080A-7) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human Rad21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rad21 antibody A300-080A (lot A300-080A-7) used for IP at 3 µg per reaction. Rad21 was also immunoprecipitated by a previous lot of this antibody (lot A300-080A-6). For blotting immunoprecipitated Rad21, A300-080A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.