www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

Rabbit anti-Rad21 Antibody, Affinity Purified

Catalogue number:
A300-080A
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£322.00 Shipping is calculated in checkout

Antibody was affinity purified using an epitope specific to Rad21 immobilized on solid support. The epitope recognized by A300-080A maps to a region between residue 575 and the C-terminus (residue 631) human Rad21 homolog using the numbering given in entry NP_006256.1 (GeneID 5885). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

CDLS4, double-strand-break repair protein rad21 homolog, hHR21, HR21, HRAD21, kleisin, MCD1, MGS, nuclear matrix protein 1, NXP1, NXP-1, protein involved in DNA double-strand break repair, RAD21 homolog, SCC1, SCC1 homolog, sister chromatid cohesion 1

Species Reactivity:

Human, Mouse

Immunogen:

between 575 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse Rad21 by we

Detection of human and mouse Rad21 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad21 antibody A300-080A (lot A300-080A-7) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human Rad21 by western blot

Detection of human Rad21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rad21 antibody A300-080A (lot A300-080A-7) used for IP at 3 µg per reaction. Rad21 was also immunoprecipitated by a previous lot of this antibody (lot A300-080A-6). For blotting immunoprecipitated Rad21, A300-080A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.