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Rabbit anti-Rad21 Antibody, Affinity Purified

Catalogue number:
Size:
100 µg
Product is available in:
  • UK
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£468.00 Estimated delivery Wed 24 Sep
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Applications:

WB, IP

Antibody Host:

Rabbit

Target:

Rad21

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

Between 581 and C-terminus

Alternative Names:

SCC1; SCC1 homolog; RAD21 homolog; protein involved in DNA double-strand break repair; NXP-1; NXP1; nuclear matrix protein 1; CDLS4; MCD1; kleisin; HRAD21; HR21; hHR21; double-strand-break repair protein rad21 homolog; sister chromatid cohesion 1; MGS

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Product Description:

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Detection of human and mouse Rad21 by we
Detection of human and mouse Rad21 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad21 antibody A300-080A (lot A300-080A-7) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human Rad21 by western blot
Detection of human Rad21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rad21 antibody A300-080A (lot A300-080A-7) used for IP at 3 µg per reaction. Rad21 was also immunoprecipitated by a previous lot of this antibody (lot A300-080A-6). For blotting immunoprecipitated Rad21, A300-080A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.