Antibody was affinity purified using an epitope specific to Diaphanous 1 immobilized on solid support. The epitope recognized by A300-077A maps to a region between residues 1 and 50 of human Diaphanous Homolog 1 using the numbering given in entry NP_005210.1 (GeneID 1729). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DFNA1, DIA1, Diaphanous-related formin-1, DRF1, hDIA1, LFHL1, protein diaphanous homolog 1, SCBMS
between 1 and 50
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human Diaphanous 1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Diaphanous 1 antibody A300-077A (lot number A300-077A-2) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Diaphanous 1 was also immunoprecipitated by a prior lot (lot number A300-077A-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).