Antibody was affinity purified using an epitope specific to SMC2 immobilized on solid support. The epitope recognized by A300-058A maps to a region between residue 1150 and the C-terminus (residue 1197) of human Structural Maintenance of Chromosomes 2 using the numbering given in entry NP_006435.1 (GeneID 10592). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CAPE, CAP-E, chromosome-associated protein E, hCAP-E, SMC protein 2, SMC-2, SMC2 (structural maintenance of chromosomes 2, yeast)-like 1, SMC2L1, structural maintenance of chromosomes (SMC) family member, chromosome-associated protein E, structural mainte
between 1150 and C-term
2 - 8°C
Detection of human SMC2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody A300-058A (lot A300-058A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human SMC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC2 antibody A300-058A (lot A300-058A-3) used for IP at 6 µg per reaction. SMC2 was also immunoprecipitated by a previous lot of this antibody A300-058A-2. For blotting immunoprecipitated SMC2, A300-058A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.