2 - 8°C
Detection of ECS-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein.Antibody: Affinity purified goat anti-ECS (DDDDK) antibody A190-101A used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of FLAG Tagged Beta-Catenin by immunofluorescence. Samples: Human microvascular endothelial cells expressing FLAG tagged beta-catenin following transient transfection.Antibody: Affinity purified goat anti-ECS (Cat. No. A190-101A) used at 1 µg/ml. Detection: FITC labeled rabbit anti-goat IgG (h+l).