Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
2 - 8°C
Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400