Antiserum was solid phase adsorbed to ensure subclass specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG1. Cross reactivity to rat IgM, IgA, IgG2a, IgG2b, and IgG2c is undetectable. Some hybridoma clones may express aberrant immunoglobulin-related peptides that are improperly recognized by this antibody.
- Applications: WB, IHC, ICC, ELISA
2 - 8°C