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SensoLyte® 520 MMP-3 Assay Kit *Fluorimetric*

Supplier:
Catalogue number:
ANA71152
Size:
1 kit
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion and other diseases is well known. MMP-3 (stromelysin-1, transin-1) is proposed as a potential therapeutic target. The SensoLyte® 520 MMP-3 Assay Kit uses a 5-FAM (fluorophore) and QXL®520 (quencher) labeled FRET peptide substrates for continuous measurement of theEnzyme activities. This substrate showed excellent specificity to MMP-3 with minimal cross-reaction with MMP-1, 2, 7, 8, 9, 13 and 14. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL®520. Upon the cleavage of the FRET peptide by MMP-3, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL®520 based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.

Product Type:

Assays & Kits

Storage Temp:

-20°C

UNSPSC Code:

41116012

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