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SensoLyte® 520 HIV Protease Assay Kit *Fluorimetric*

Supplier:
Catalogue number:
ANA71147
Size:
1 kit
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£665.00 Shipping is calculated in checkout

The SensoLyte® 520 HIV Assay Kit uses a new FRET peptide substrate that incorporates HiLyte™ Fluor 488 (fluorophore) and QXL® 520 (quencher) for continuous measurement ofEnzyme activities. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL® 520. Upon cleavage of the FRET peptide by HIV protease, the fluorescence of HiLyte™ Fluor 488 is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this HiLyte™ Fluor 488/QXL® 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components, thus providing better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format. The kit contains: HiLyte™ Fluor 488/QXL® 520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage), Assay buffer, HIV protease inhibitor, Fluorescence reference standard for calibration, A detailed protocol

Product Type:

Assays & Kits

UNSPSC Code:

41116012