Tel: +44 (0)1223 316 855
life science research products, biological research products, biotechnology
close ...


Supplier:
Product types:
Gibson Assembly® Kits
Over 40,000 Peptides & Proteins Available
Find Your Peptide Simply with Our Peptide Search

Assemble DNA Constructs Simply & Efficiently Using The Gibson Assembly® Method

SensoLyte® 520 MMP-13 Assay Kit *Fluorimetric* - ANA71156 - 1 kit - AnaSpec - assays & kits

SensoLyte® 520 MMP-13 Assay Kit *Fluorimetric*
print page
Size: 1 kit
£650.00
GBP | Euro | USD
Add to basket
Catalogue number:
ANA71156
Name:
SensoLyte® 520 MMP-13 Assay Kit *Fluorimetric*
Supplier:
Availability:

Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMP-13 (collagenase-3, rat collagenase) is involved in quite a few diseases such as arthritis and asthma. The SensoLyte® 520 MMP-13 Assay Kit uses a 5-FAM (fluorophore) and QXL® 520 (quencher) labeled FRET peptide substrates for continuous measurement of theEnzyme activities. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL® 520. Upon the cleavage of the FRET peptide by MMP-13, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL® 520 based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format. Members of the MMP family have poor substrate sequence specificity, making it difficult to use a peptide substrate alone to differentiate the activity of a particular MMP from other MMPs. If several MMPs are coexisting in your samples and you would like to specifically measure MMP-13 activity, please choose the SensoLyte® Plus MMP-13 Assay Kit, Cat# 72019.

Data sheet:

Click to view / download

Product Type:
Assays & Kits
Storage Temp:
-20°C
UNSPSC Code:
41116012
Contact Our Friendly Technical Team