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SensoLyte® 520 HCV Protease Assay Kit *Fluorimetric* - ANA71145 - 1 kit - AnaSpec - assays & kits

SensoLyte® 520 HCV Protease Assay Kit *Fluorimetric*
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SensoLyte® 520 HCV Protease Assay Kit *Fluorimetric*

The SensoLyte® 520 HCV Assay Kit uses a new FRET peptide substrate that incorporates 5-FAM (fluorophore) and QXL® 520 (quencher) for a continuous measurement ofEnzyme activities. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL® 520. Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluorescence of 5-FAM is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this 5-FAM/QXL® 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components. This assay is ten times more sensitive than an EDANS/DABCYL based assay and can detect 0.1 pmole of HCV NS3/4a protease. The assays are performed in a convenient 96-well or 384-well microplate format. The kit contains: 5-FAM/QXL® 520-based FRET peptide substrate (Ex/Em=490/520 nm upon cleavage), Fluorescence reference standard for calibration, A detailed protocol

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