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  2. Bethyl Laboratories, Inc.
  3. Rabbit anti-CD28 Antibody, Affinity Purified
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Rabbit anti-CD28 Antibody, Affinity Purified

Catalogue number:
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A304-418A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£468.00
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Estimated delivery Wed 5 Nov
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Alternative Names:

CD28 antigen; CD antigen CD28; Tp44; T-cell-specific surface glycoprotein CD28

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Product Description:

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Detection of human CD28 by western blot.
Detection of human CD28 by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD28 antibody A304-418A (lot A304-418A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human CD28 by western blot
Detection of human CD28 by western blot of immunoprecipitates. Samples: Whole cell lysate (50 µg) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD28 antibody A304-418A (lot A304-418A-1) used for IP at 6 µg per reaction. CD28 was also immunoprecipitated by rabbit anti-CD28 antibody BL15921. For blotting immunoprecipitated CD28, A304-418A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.