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  3. Rabbit anti-CD45 Antibody, Affinity Purified
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Rabbit anti-CD45 Antibody, Affinity Purified

Catalogue number:
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A304-376A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µg
Product is available in:
  • UK
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£468.00
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Estimated delivery Fri 7 Nov
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Alternative Names:

L-CA; protein tyrosine phosphatase, receptor type, c polypeptide; T200 leukocyte common antigen; T200 glycoprotein; receptor-type tyrosine-protein phosphatase C; LY5; Leukocyte common antigen; GP180; CD45R; B220; CD45 antigen; LCA; CD45; CD antigen CD45;

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Product Description:

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Detection of human CD45 by western blot.
Detection of human CD45 by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN or RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD45 antibody A304-376A (lot A304-376A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human CD45 by western blot
Detection of human CD45 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD45 antibody A304-376A (lot A304-376A-1) used for IP at 6 µg per reaction. CD45 was also immunoprecipitated by rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (A700-012). For blotting immunoprecipitated CD45, A700-012 was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.