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  3. Rabbit anti-CD45 Antibody, Affinity Purified
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Rabbit anti-CD45 Antibody, Affinity Purified

Catalogue number:
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A304-376A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00
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Estimated delivery Wed 22 May
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Applications:

WB, IP, IHC, ICC-IF

Antibody Host:

Rabbit

Target:

CD45

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

Between 1254 and 1304

Alternative Names:

L-CA; protein tyrosine phosphatase, receptor type, c polypeptide; T200 leukocyte common antigen; T200 glycoprotein; receptor-type tyrosine-protein phosphatase C; LY5; Leukocyte common antigen; GP180; CD45R; B220; CD45 antigen; LCA; CD45; CD antigen CD45;

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Product Description:

Rabbit anti-CD45 Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human CD45 by western blot.
Detection of human CD45 by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN or RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD45 antibody A304-376A (lot A304-376A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human CD45 by western blot
Detection of human CD45 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD45 antibody A304-376A (lot A304-376A-1) used for IP at 6 µg per reaction. CD45 was also immunoprecipitated by rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (A700-012). For blotting immunoprecipitated CD45, A700-012 was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.
Detection of human CD45 by immunocytoche
Detection of human CD45 by immunocytochemistry. Samples: A: Acetone-fixed Jurkat cells, B:Formaldehyde-fixed Jurkat cells, C: Acetone-fixed Ramos cells, and D: Formaldehyde-fixed Ramos cells. Antibody: Affinity purified rabbit anti- CD45 (Cat. No.A304-376A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight® 594 Conjugated (Cat. No. A120-201D4) used at a dilution of 1:100.
Detection of human CD45 by immunohistoch
Detection of human CD45 by immunohistochemistry. Sample: FFPE section of human lung cancer infiltrate. Antibody: Affinity purified rabbit anti- CD45 (Cat. No. A304-376A Lot1) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB
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