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  3. A304-407A
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Rabbit anti-Phospho RNA Polymerase II (S2) Antibody Affinity Purified

Catalogue number:
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A304-407A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µl (1000 µg/ml)
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£498.00
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Estimated delivery Fri 19 Dec
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Applications:

IHC,IP,WB

Antibody Host:

Rabbit

Target:

Phospho RNA Polymerase II (S2)

Species Reactivity:

Human;Mouse

Immunogen:

Surrounding serine 2

Alternative Names:

DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; hRPB220; hsRPB1; POLR2; POLRA; polyme

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Product Description:

Rabbit anti-Phospho RNA Polymerase II (S2) Antibody Affinity Purified - 100 µl (1000 µg/ml)

Detection of human Phospho RNA Polymeras
Detection of human Phospho RNA Polymerase II (S2) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: RNA Polymerase II was immunoprecipitated using rabbit anti-RNA Polymerase II antibody A300-653A. Following immunoprecipitation the product of the IP reaction was mock treated (-) or treated with phosphatases (+). Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody A304-407A was used for WB at 1 µg/ml (upper panel). To examine total RNA Polymerase II, the blot was stripped and then blotted with rabbit anti-RNA Polymerase II antibody A300-653A (lower panel). Detection: Chemiluminescence with exposure times of 30 seconds (upper panel) and 15 seconds (lower panel).
Detection of human Phospho RNA Polymeras
Detection of human Phospho RNA Polymerase II (S2) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody A304-407A (lot A304-407A-1) used for IP at 6 µg per reaction. Phospho RNA Polymerase II was also immunoprecipitated by rabbit anti-Phospho RNA Polymerase II antibodies A304-408A, A300-654A and A300-655A, and rabbit anti-RNA Polymerase II antibody A300-653A. For blotting immunoprecipitated Phospho RNA Polymerase II, A300-653 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.