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  3. A304-405A
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Rabbit anti-RNA Polymerase II Antibody, Affinity Purified

Catalogue number:
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A304-405A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µg
Product is available in:
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£468.00
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Estimated delivery Wed 24 Sep
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Applications:

IP, ChIP-PCR

Antibody Host:

Rabbit

Target:

RNA Polymerase II

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

Between 775 and 825

Alternative Names:

RPBh1; RPOL2; RpIILS; RPB1; RNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; polymerase (RNA) II subunit A; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; POLR2; hsRPB1; hRPB220; DNA-directed RNA polymerase III largest

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Product Description:

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Detection of human RNA Polymerase II by
Detection of human RNA Polymerase II by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-RNA Polymerase II antibody A304-405A (lot A304-405A-1) used for IP at 6 µg per reaction. RNA Polymerase II was also immunoprecipitated by rabbit anti-RNA Polymerase II antibody BL16638. For blotting immunoprecipitated RNA Polymerase II, A300-653A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Binding at pause site of the Jun B gene.
Binding at pause site of the Jun B gene. Chromatin isolated from one 15 cm plate of HeLa cells was immunoprecipitated with 8.0 µg of anti-RNA polymerase II antibodies and PCR amplified.kerning24