Antibody was affinity purified using an epitope specific to CD7 immobilized on solid support. The epitope recognized by A304-396A maps to a region between residue 190 to 240 of human CD7 Molecule using the numbering given in entry NP_006128.1 (GeneID 924). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CD antigen CD7, CD7 antigen (p41), GP40, LEU-9, p41 protein, T-cell antigen CD7, T-cell leukemia antigen, T-cell surface antigen Leu-9, Tp40, TP41
Between 190 and 240
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human CD7 by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN or RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD7 antibody A304-396A (lot A304-396A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human CD7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD7 antibody A304-396A (lot A304-396A-1) used for IP at 6 µg per reaction. For blotting immunoprecipitated CD7, A304-396A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.