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Rabbit anti-PolD2 Antibody, Affinity Purified

Catalogue number:
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£468.00 Estimated delivery Wed 24 Sep
Shipping is calculated in checkout
Applications:

WB, IP, IHC

Antibody Host:

Rabbit

Target:

PolD2

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

Between 419 and C-terminus

Alternative Names:

DAN polymerase delta 2, accessory subunit; DNA polymerase delta subunit 2; DNA polymerase delta subunit p50; Pol delta B subunit (p50); polymerase (DNA directed), delta 2, accessory subunit; polymerase (DNA directed), delta 2, regulatory subunit 50kDa; po

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Product Description:

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Detection of human PolD2 by western blot
Detection of human PolD2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PolD2 antibody A304-322A (lot A304-322A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human PolD2 by western blot
Detection of human PolD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PolD2 antibody A304-322A (lot A304-322A-1) used for IP at 6 µg per reaction. PolD2 was also immunoprecipitated by rabbit anti-PolD2 antibody BL15767. For blotting immunoprecipitated PolD2, A304-322A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.