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Rabbit anti-CD3E Antibody, Affinity Purified

Catalogue number:
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£468.00 Estimated delivery Fri 12 Sep
Shipping is calculated in checkout
Applications:

WB, IP, IHC

Antibody Host:

Rabbit

Target:

CD3E

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

Between 157 and C-terminus

Alternative Names:

CD3-epsilon; TCRE; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; CD antigen CD3e; IMD18; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilo

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Product Description:

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Detection of human CD3E by western blot.
Detection of human CD3E by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD3E antibody A304-291A (lot A304-291A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human CD3E by western blot
Detection of human CD3E by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD3E antibody A304-291A (lot A304-291A-1) used for IP at 6 µg per reaction. CD3E was also immunoprecipitated by rabbit anti-CD3E antibody BL16032 and rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (A700-016). For blotting immunoprecipitated CD3E, A700-016 was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.