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Rabbit anti-PFKM Antibody, Affinity Purified

Catalogue number:
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
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£468.00 Estimated delivery Wed 24 Sep
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Applications:

WB, IP

Antibody Host:

Rabbit

Target:

PFKM

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

Between 1 and 50

Alternative Names:

PFK-M; PPP1R122; phosphohexokinase; 6-phosphofructokinase, muscle type; phosphofructokinase-M; protein phosphatase 1, regulatory subunit 122; phosphofructokinase, polypeptide X; phosphofructokinase 1; phosphofructo-1-kinase isozyme A; PFKX; PFKA; PFK-1; P

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Product Description:

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Detection of human and mouse PFKM by wes
Detection of human and mouse PFKM by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-PFKM antibody A304-255A (lot A304-255A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human PFKM by western blot
Detection of human PFKM by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PFKM antibody A304-255A (lot A304-255A-1) used for IP at 6 µg per reaction. PFKM was also immunoprecipitated by rabbit anti-PFKM antibody A304-256A. For blotting immunoprecipitated PFKM, A304-255A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.