MADS box transcription enhancer factor 2, polypeptide D, myocyte-specific enhancer factor 2D
between 425 and 475
2 - 8°C
Detection of human MEF2D by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-MEF2D antibody A303-521A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. MEF2D was also immunoprecipitated by rabbit anti-MEF2D antibody A303-522A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Detection of human MEF2D by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- MEF2D (Cat. No. A303-521A Lot1, left image and Cat. No. A303-522A Lot1, right image) used at a dilution of 1:200 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).