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Rabbit anti-c-Kit Antibody, Affinity Purified

Catalogue number:
A303-252A
Size:
100 µl
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£337.00 Estimated delivery Fri 25 Jun
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Antibody was affinity purified using an epitope specific to c-Kit immobilized on solid support. The epitope recognized by A303-252A maps to a region between residue 925 and 976 of c-Kit using the numbering given in entry NP_000213.1 (GeneID 3815). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Product Type:

Antibodies

Alternative Names:

CD antigen CD117, CD117, C-Kit, c-Kit protooncogene, mast/stem cell growth factor receptor Kit, MASTC, p145 c-kit, PBT, piebald trait protein, proto-oncogene c-Kit, proto-oncogene tyrosine-protein kinase Kit, SCFR, soluble KIT variant 1, tyrosine-protein

Species Reactivity:

Human

Immunogen:

between 925 and 976

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Formulation:

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human c-Kit by western blot

Detection of human c-Kit by western blot and immunoprecipitation. Samples: Whole cell lysate from human erythroleukemia (HEL) cells (50, 15, and 5 µg for WB; 1 mg for IP, 20% of IP loaded and HEK293T (T; 50 µg). Antibodies: Affinity purified rabbit anti-c-Kit antibody A303-252A used for WB at 0.1 µg/ml (A) and 1.0 µg/ml (B) and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Detection of human c-Kit by immunohistoc

Detection of human c-Kit by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- c-Kit (Cat. No. A303-252A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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