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Rabbit anti-ICAM-1 Antibody, Affinity Purified

Catalogue number:
A302-874A
Size:
100 µl
Product is available in:
  • UK
  • Ireland
  • Europe
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  • Rest of World
£337.00 Estimated delivery Fri 25 Jun
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Antibody was affinity purified using an epitope specific to ICAM-1 immobilized on solid support. The epitope recognized by A302-874A maps to a region between residue 467 and 517 of human Intercellular Adhesion Molecule 1 using the numbering given in entry NP_000192.2 (GeneID 3383). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Product Type:

Antibodies

Alternative Names:

BB2, CD antigen CD54, CD54, cell surface glycoprotein P3.58, epididymis secretory sperm binding protein, ICAM-1, intercellular adhesion molecule 1, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, major group rhinovirus receptor, P3.58

Species Reactivity:

Human

Immunogen:

between 467 and 517

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Formulation:

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human ICAM-1 by western blo

Detection of human ICAM-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ICAM-1 antibody A302-874A used for IP at 3 µg/mg lysate. ICAM-1 was also immunoprecipitated by rabbit anti-ICAM-1 antibody A302-873A, which recognizes an upstream epitope. For blotting immunoprecipitated ICAM-1, A302-873A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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