Antibody was affinity purified using an epitope specific to ICAM-1 immobilized on solid support. The epitope recognized by A302-874A maps to a region between residue 467 and 517 of human Intercellular Adhesion Molecule 1 using the numbering given in entry NP_000192.2 (GeneID 3383). Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BB2, CD antigen CD54, CD54, cell surface glycoprotein P3.58, epididymis secretory sperm binding protein, ICAM-1, intercellular adhesion molecule 1, intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, major group rhinovirus receptor, P3.58
between 467 and 517
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human ICAM-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ICAM-1 antibody A302-874A used for IP at 3 µg/mg lysate. ICAM-1 was also immunoprecipitated by rabbit anti-ICAM-1 antibody A302-873A, which recognizes an upstream epitope. For blotting immunoprecipitated ICAM-1, A302-873A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.