The Bisulfite Conversion Kit simplifies the analysis of DNA methylation by providing optimised conversion reagents and an easy-to-use protocol. DNA of interest is rapidly heat denatured in a thermocycler in the presence of the bisulfite conversion reagent and a DNA denaturant. The conversion reaction can be performed in as little as 1.5 hours with a 99% conversion efficiency of unmethylated cytosines to uracils. After DNA conversion, the sample is added to the included DNA purification columns, and a simple, on-column desulfonation is performed. Ready-to-use DNA is then eluted from the columns. For your convenience, a positive control conversion-specific PCR primer pair that is specific for bisulfite converted human and mouse DNA is included. Because the primer pair produces a PCR product only if conversion has occurred, you can validate the success of the conversion reaction before spending extra time and money on sequencing.
