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QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated p24 ELISA)

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Size:
96 assays _$$_
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£959.00 Estimated delivery Wed 31 Jul
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Measuring the HIV-1 p24 antigen is a long-established method for lentivirus quantitation. However, the traditional p24 ELISA detects both virus-associated p24 and free p24 generated by 293 cells during transient transfection. Free p24 can account for a substantial portion of total p24 in the supernatant. Therefore, the ELISA typically overestimates the quantity of lentivirus present. Our QuickTiter™ Lentivirus Titer Kit (Lentivirus-Associated HIV p24) substantially minimizes this problem. A proprietary technology separates the lentivirus from free p24 in solution prior to running the ELISA portion of the assay.

Storage Temperature:

Upon receipt, aliquot and store the Recombinant HIV-1 p24 Standard at -20°C to avoid multiple freeze

Assay Principle for the QuickTiter™ Lent
Assay Principle for the QuickTiter™ Lentivirus Titer Kit. Lentivirus particles are packaged with p24 protein, but additional overexpressed p24 protein is present in viral supernatants. Traditional p24 ELISA kits detect both sources of p24 which overestimates viral titer. The QuickTiter™ Lentivirus Titer Kit uses technology to pull the virus out of solution, while leaving the free p24 in solution, yielding a more accurate viral titer.
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