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Unique Cryopreservation Media

Exo-Urine™ EV Isolation Kit

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Stellaris® RNA FISH Technology

The Stellaris® RNA FISH technology offers an RNA visualisation method for simultaneous detection, localisation, and quantification of individual mRNA molecules at the cellular level. Both highly sensitive and specific, a Stellaris® RNA FISH assay is comprised of up to 48 single-labelled oligonucleotides designed to selectively bind to target RNA transcripts. When bound to RNA, a fluorescent signal is produced for the detection of single RNA molecules as diffraction-limited spots using conventional fluorescence microscopy. The inherent redundancies of this direct detection method minimize the possibility of false negative and false positive signals. Positive signals are identified from combined localised fluorescence of multiple probes. Off-target binding of single probes generates only weak and diffuse fluorescence, which falls well below the threshold for detection of the targeted mRNAs. The short probe lengths can easily penetrate the cellular matrix for efficient hybridisation.

Benefits Of Using The Stellaris® FISH Technology
• Simple protocol
• Inexpensive
• Platform-independent
• Same-day results
• Compatible with many sample types & applications
• Multiplex RNA target detection
• No isolation, purification or amplification of RNA required

Wide Variety Of Fluorophores AvailableStellaris® RNA FISH Probes Available In A Wide Variety Of Fluorophores
Stellaris® RNA FISH probes are available in a wide variety of fluorophores in the visible spectrum, including LGC Biosearch’s CAL Fluor and Quasar dyes (CAL Fluor® Orange 560, Quasar® 570, CAL Fluor® Red 590, CAL Fluor® Red 610, CAL Fluor® Red 635, Quasar® 670) and can be used in a multiplex assay.

The dye choices in a multiplex assay are largely determined by the filters available on the imaging platform, but it is certainly possible to design an assay including a DAPI nuclear stain and three Stellaris® RNA FISH probe sets labelled with reporter dyes with minimal spectral overlap.

Portfolio Available
1. Stellaris® FISH Custom Probe Sets
Design a custom set to detect your RNA of interest to allow for visualisation, localisation, and quantification of individual mRNA transcripts
2. Stellaris® DesignReady Probe Sets
Rigorous pre- and post-design bioinformatic analysis to maximise specificity and most are available with the following dyes: CAL Fluor® Orange 560, Quasar® 570, CAL Fluor® Red 590, CAL Fluor® Red 610, CAL Fluor® Red 635, Quasar® 670 and FAM
3. Stellaris® Positive Control Probe Set
Convenient, ready-to-ship positive controls for reference and long non-coding RNA targets
4. Stellaris® RNA FISH Buffers
Ideal for performing Stellaris® RNA FISH on cells and tissue, as well as other sample types. The buffers contain proprietary additives to enhance signal and reduce background in assays that may typically exhibit more pronounced background fluorescence

Applications
• Cancer targets
• Neuroscience
• Long non-coding RNA
• Pathogen detection & screening

Find Out More
Click on the appropriate link below or contact our LGC Biosearch Technologies specialist

Speak To Our LGC Biosearch Technologies Specialist Doaa

LGC Biosearch Technologies
Tonbo Biosciences