Stellaris RNA FISH buffers

Stellaris® RNA FISH buffers from LGC Biosearch Technologies contain proprietary additives to enhance detection with Stellaris RNA FISH probe sets, particularly for applications that typically exhibit more pronounced background fluorescence.

Stellaris RNA FISH buffers outperform conventional RNA FISH buffers

A direct comparison showing background reduction by using Stellaris RNA FISH Buffers (Cat#: SMF-HB1-10, SMF-WA1-60, SMF-WB1-20). Following the adherent cell protocol, formalin fixed human cells were probed with Human POLR2A (Cat# VSMF-2293-5). Left Panel: RNA FISH performed with Stellaris RNA FISH Buffers. Right Panel: RNA FISH performed with Conventional RNA FISH buffer recipes. Note: Results may vary according to original probe set performance and sample type. 

How the Stellaris FISH technology works
When bound to RNA, a fluorescent signal is produced for the detection of single RNA molecules as diffraction-limited spots using conventional fluorescence microscopy. The inherent redundancies of this direct detection method minimize the possibility of false negative and false positive signals. Positive signals are identified from combined localised fluorescence of multiple probes. Off-target binding of single probes generates only weak and diffuse fluorescence, which falls well below the threshold for detection of the targeted mRNAs. The short probe lengths can easily penetrate the cellular matrix for efficient hybridisation.

Benefits of using the Stellaris FISH technology
• Simple protocol
• Inexpensive
• Platform-independent
• Same-day results
• Compatible with many sample types and applications
• Multiplex RNA target detection
• No isolation, purification or amplification of RNA required

Find out more about the full Stellaris FISH technology portfolio