The Stellaris® RNA FISH technology from LGC Biosearch Technologies offers an RNA visualisation method for simultaneous detection, localisation, and quantification of individual mRNA molecules at the cellular level. Both highly sensitive and specific, a Stellaris RNA FISH assay is comprised of up to 48 single-labelled oligonucleotides designed to selectively bind to target RNA transcripts.
How the Stellaris FISH technology works
When bound to RNA, a fluorescent signal is produced for the detection of single RNA molecules as diffraction-limited spots using conventional fluorescence microscopy. The inherent redundancies of this direct detection method minimise the possibility of false negative and false positive signals. Positive signals are identified from combined localised fluorescence of multiple probes. Off-target binding of single probes generates only weak and diffuse fluorescence, which falls well below the threshold for detection of the targeted mRNAs. The short probe lengths can easily penetrate the cellular matrix for efficient hybridisation.
Benefits of using the Stellaris FISH technology
• Simple protocol
• Same-day results
• Compatible with many sample types and applications
• Multiplex RNA target detection
• No isolation, purification or amplification of RNA required
Stellaris DesignReady RNA FISH probe sets
Professionally designed with rigorous pre- and post-design bioinformatic analysis to maximise specificity. Most Stellaris DesignReady RNA FISH probe sets are available in the following dyes:
• CAL Fluor® Red 590
• Quasar® 570
• CAL Fluor Red 610
• Quasar 670
• CAL Fluor Red 635
• CAL Fluor Orange 560
A small selection of the probes available are attached below. To find the Stellaris DesignReady RNA FISH probe sets you need, complete the contact form.
Pre-designed RNA FISH probe sets include: