The Stellaris® RNA FISH technology from LGC Biosearch Technologies offers an RNA visualisation method for simultaneous detection, localisation, and quantification of individual mRNA molecules at the cellular level. Both highly sensitive and specific, a Stellaris RNA FISH assay is comprised of up to 48 single-labelled oligonucleotides designed to selectively bind to target RNA transcripts.
How the Stellaris FISH technology works
When bound to RNA, a fluorescent signal is produced for the detection of single RNA molecules as diffraction-limited spots using conventional fluorescence microscopy. The inherent redundancies of this direct detection method minimize the possibility of false negative and false positive signals. Positive signals are identified from combined localised fluorescence of multiple probes. Off-target binding of single probes generates only weak and diffuse fluorescence, which falls well below the threshold for detection of the targeted mRNAs. The short probe lengths can easily penetrate the cellular matrix for efficient hybridisation.
Benefits of using the Stellaris FISH technology
• Simple protocol
• Same-day results
• Compatible with many sample types and applications
• Multiplex RNA target detection
• No isolation, purification or amplification of RNA required
Stellaris RNA FISH custom probe sets
Using custom probe sets enables the researcher to design their own to detect the RNA of interest.
How to order customised Stellaris RNA FISH probe sets
The corresponding target sequences are required. If the probe set has not yet been designed, contact us with the target sequences (accession number and description of the target of interest).
Please select the appropriate fluorophore from the table below that best matches the filter set of your fluorescence microscope.
NB: Due to variable auto-fluorescence in the green channel inherent to cells and tissue, it is not recommended to use the FAM (fluorescein) for some Stellaris RNA FISH applications. It is recommended to test the sample for green channel autofluorescence before ordering FAM-labelled sets or ordering an alternative dye that is compatible with the experiment.