Full-length amplicon sequencing services

Full-length amplicon sequencing provides unrivalled taxonomic species-level resolution by sequencing the full rRNA gene, not just the hypervariable regions within it. rRNA genes range in size from 1-2kb, sizes short-read platforms cannot capture, so the resulting amplicons are sequenced using the latest PacBio Revio platform. This method allows for the identification and resolution of closely related species that would not be resolved with other metagenomic sequencing methods. Zymo is a PacBio certified service provider.

What you can expect from Zymo’s services

• Quick turnaround time: results in as little as 3 weeks
• Unrivalled species resolution
• Consultation and one-to-one support
• Unrivalled data quality
• Stringent QC using microbial standards
• Reference database curated in-house

Comprehensive bioinformatics analysis report

The full-length 16S rRNA sequencing service report provides compositional bar plots, composition heatmaps, alpha-diversity and beta diversity plots, and statistical group comparison with LEfSe.

Continuous monitoring for standardisation and consistency

Zymo’s full-length 16S sequencing pipeline results in the same community profile as expected for their Microbial Community DNA Standard. The pipeline is consistently monitored with Zymo’s own community standards, which have been cited in over 1000 publications and have helped the microbiomics research community move towards standardisation and reproducibility.

Full-length amplicon sequencing technical specifications