ATAC-seq services

Assay for Transposase-Accessible Chromatin (ATAC-seq) is a method used to map open chromatin sites at a genome-wide level. Open chromatin, also known as euchromatin, is where regulatory elements such as promoters, enhancers, and transcription factors are active and have access to DNA. This method allows for the analysis of what epigenetic mechanisms are at play in your samples without needing prior knowledge of these mechanisms.

ATAC-seq uses the adaptor-loaded Tn5 hyperactive transposase to fragment DNA at open chromatin sites and integrate sequencing adaptors at these sites. The generated library is then subjected to next-generation sequencing so that the regions of the genome with accessible chromatin can be analysed. Cambridge Bioscience's ATAC-seq servcie are provided by Active Motif. 

Active Motif’s ATAC-seq services

• Compatible with input as low as 50,000 cells
• Detection of differentially open regions
• High levels of standardization and reproducibility
• High FriP score and peak number

Applications of ATAC-seq

• Compare genome-wide open chromatin signatures across patient populations
• Identify epigenetic mechanisms driving disease such as transcription factors and other gene regulatory elements
• Analyse treatment response ins gene regulation mechanisms
• Develop models of dynamic chromatin reorganization during different stages of cell differentiation and development

Material for download

Active Motif epigenetics products and services brochure