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Microbiomics analysis: PCR and NGS

Whilst PCR can be sufficient for looking at a small number of microbial species or working with cultured samples, next generation sequencing (NGS) has become the method of choice for many researchers working in metagenomics.

Options are available to sequence the highly variable 16s, 18s and ITS amplicons to profile metagenomics samples or to use shotgun sequencing to learn more about the genomes of the organisms in a sample for functional profiling. Shotgun metagenomics also allows you to learn more about novel organisms that might not be in the reference database.