High-throughput DNA library preparation kit: ExpressPlex

The ExpressPlex library preparation kits from seqWell enable researchers to increase their sequencing throughput, offering significant time saving with libraries prepared and on the sequencer within just half a day. With a short, 90-minute protocol for 96 samples, ExpressPlex achieves consistent read-depths without individual normalisation, offering a simplified workflow which is well-suited for manual, automated and ultra-high throughput library preparation.

Benefits of using the ExpressPlex library preparation kit
•    90-minute library prep method (for 96 samples)
•    30 minutes or less hands-on-time
•    Prep and sequence up to 6144 samples within 24 hours
•    Fragmentation, barcoding and amplification in just one step
•    Save significant time, cost and plastic
•    Designed for support of automation
•    Plate resealing option available

ExpressPlex provides a simplified workflow

Individual normalisation is no longer required to achieve more consistent read-depths with ExpressPlex

Libraries were prepared from three different reference plasmids of varying sizes across a 10-fold input range at full reaction volume for ExpressPlex (16 µL) and Nextera™ XT (50 µL). For each method, inputs ranged from 4-40ng for ExpressPlex (standard input is 16ng) and 0.4 – 4ng for Nextera™ XT (standard input is 1ng).

Success rates of plasmid assembly are higher using ExpressPlex

Libraries were prepared, sequenced (MiSeq™ Nano; 2 x150) and de novo assembled using seqWell’s SNAP plasmid assembly pipeline. The sequencing run was downsampled to either 1,000 or 500 average read pairs per sample to identify error-free, circular plasmid assemblies. Even at only 500 average read pairs all ExpressPlex samples generated correct circularized assemblies. At the same number of reads, however, Nextera™ XT (full and ¼ reactions) failed 8-10% of assembly attempts.

Material available to download
ExpressPlex Brochure
ExpressPlex Data Sheet

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