High-throughput DNA library preparation kit: ExpressPlex 2.0

The latest ExpressPlex 2.0 library preparation kit from seqWell is now powered by TnX, supporting reduced bias and uniform coverage for better sequencing. The upgraded kit enables researchers to increase their sequencing throughput, offering significant time saving with libraries prepared and on the sequencer within just half a day. With a short, 100-minute protocol for 96 samples, ExpressPlex achieves consistent read-depths without individual normalisation, offering a simplified workflow which is well-suited for manual, automated and ultra-high throughput library preparation.

Benefits of using the ExpressPlex library preparation kit

• Improved performance: TnX transposase for reduced bias and improved coverage uniformity
• 100-minute library prep method (for 96 samples)
• 30 minutes or less hands-on-time
• Prep and sequence up to 6144 samples within 24 hours
• Fragmentation, barcoding and amplification in just one step
• Designed for automation

Applications of ExpressPlex 2.0

• Gene editing QC: ideal for quality control in CRISPR-based gene editing, enabling highly reproducible and efficient library prep for downstream sequencing. Compatible with various assays like UDiTaS™, ATAC-Seq, SHARE-Seq, GUIDE-tag, CHANGE-Seq, and RGen-Seq for evaluating genomic alterations.
• Targeted sequencing: supports targeted sequencing applications, including mutation detection and genetic variant analysis, with reduced hands-on time and greater accuracy.
• Small genome sequencing: perfect for sequencing small genomes (e.g., plasmids, viruses) with improved accuracy and throughput.
• Metagenomics: facilitates metagenomic profiling by efficiently preparing DNA libraries for sequencing of complex microbial communities, enhancing microbial diversity insights.
• Amplicon sequencing: enables streamlined preparation of amplicon libraries for sequencing targeted genomic regions in clinical diagnostics and research.

Compatibility with Beckman Coulter's Echo Acoustic Liquid Handling System

• Fully automated, ultra-high throughput NGS library prep
• Sample to sequencer in ~ 2 hours
• Miniaturisation to 3 µl
• 384-well format
• Plasmid sequencing economies of scale
• TnX-driven uniformity of coverage

ExpressPlex provides a simplified workflow

Individual normalisation is no longer required to achieve more consistent read-depths with ExpressPlex 2.0

Libraries were prepared from three different reference plasmids of varying sizes across a 10-fold input range at full reaction volume for ExpressPlex (16 µL) and Nextera™ XT (50 µL). For each method, inputs ranged from 4-40ng for ExpressPlex (standard input is 16ng) and 0.4 – 4ng for Nextera™ XT (standard input is 1ng).

ExpressPlex 2.0 provides uniform coverage for enhanced sequencing

ExpressPlex 2.0 stands out as the only library prep kit driven by TnX technology. Designed to minimise insertional bias, TnX enhances coverage uniformity for superior sequencing results. In a direct comparison with a Tn5-based competitor kit for sequencing two distinct plasmids, ExpressPlex 2.0 delivered significantly lower coefficient of variation (CV) values and a more consistent coverage range (max/min).

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