Quick-ITS sequencing library preparation

The Quick-ITS Plus NGS Library Prep kit is the most streamlined next-generation sequencing (NGS) library prep kit to target the fungal ITS region, with only 30 minutes of hand-on time for 96 samples. Featuring a simple and fast workflow, the Quick-ITS Plus NGS Library Prep kit combines targeted amplification of the microbial genome and barcode addition, without the need for additional library quantification analysis.

The Quick-ITS Plus NGS Library Prep kit is compatible with Illumina® MiSeq® without the need to add custom sequencing primers. 

Benefits of using the Quick-ITS Plus NGS Library Prep kit
• Rapid and targeted fungal community profiling
• Automation ready, with no need for normalisation due to a single PCR step
• Real-time PCR for absolute microbial copy number quantification. 
• Compatible with microbial samples as low as DNA ≤100 ng

The Quick-ITS Plus NGS Library Prep Kit offers the fastest ITS workflow 

The Quick-ITS workflow versus the Illumina® metagenomic protocol. Total hands-on time calculation is based on the preparation of 96 DNA samples. 

Normalisation free
There is no need to control sample input or PCR cycles. The workflow auto-normalizes and produces similar amounts of sequencing reads across all samples regardless of different DNA inputs.

Figure 2. The Quick-ITS™ Plus NGS Library Prep Kit results in similar amounts of reads across different input amounts without normalization. 0.01-100 ng ZymoBIOMICS™ Microbial Community Standard and soil DNA were used as inputs. Libraries were pooled by equal volumes (2 µl each) without further normalization and sequenced using the MiSeq® Reagent Kit v3 (600-cycle). The CV (coefficient of variation) is the ratio of the standard deviation to the mean with lower values corresponding to less dispersion around the mean.

Reduce PCR Chimera Formation
Up to 70% chimera reduction without controlling PCR cycles. Equal amounts of ZymoBIOMICS™ Microbial Community DNA Standard and soil DNA were used as inputs and amplified for 42 cycles using 1-step PCR and Equalase™ compared to using the Illumina metagenomic Protocol. Chimeric sequences were predicted with UCHIME.

Material available for download
Quick-ITS Plus NGS Library Prep Kit datasheet
Quick-ITS Plus NGS Library Prep Kit plate setup guide
Quick-ITS Plus NGS Library Prep Kit protocol
Quick-ITS Plus NGS Library Prep Kit safety datasheet

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