Zymo-Seq Trio WGBS library prep kit

The Zymo-Seq Trio WGBS library prep kit provides efficient WGBS library preparation for small DNA fragments such as cfDNA, with an input range of 5-10ng. The workflow consist of 3 steps: 1. Bisulphite conversion; 2. Adapter ligation; 3. Index PCR amplification.

The bisulfite conversion step uses the EZ DNA Methylation Lightning reagent, which is gentle on already-fragmented DNA, converting over 99.5% of unmethylated cytosines and protecting over 99.5% of methylated cytosines. The direct ligation step of the protocol ensures the process is optimised for small fragments, capturing their ends for full sequencing coverage. This preservation of native termini also guarantees accurate methylation calling. The libraries produced after PCR amplification are compatible with all Illumina sequencing platforms.

Benefits of the Zymo Trio WGBS kit

• True end detection
• Accurate methylation calling
• Simple three-step workflow
• Gentle bisulfite conversion and protection of methylated cytosines

Material for download

Zymo Trio WGBS kit protocol

Zymo Trio WGBS kit datasheet