Small RNA quantification kit: QuantiMir

The sensitive and robust QuantiMir™ kit from System Biosciences (SBI) enables the generation of PCR-ready cDNA from just 10 pg of total RNA. With a simple protocol, the QuantiMir kit can be used for the profiling of any small RNA and is more sensitive, quantitative and easier to perform than Northern or RNase protection experiments.

Benefits of using the QuantiMir kit
• Simple and robust procedure
• Rapidly and efficiently convert all small RNAs into cDNAs for qPCR
• Suitable for high-throughput screening of clinical samples
• Sensitive and accurate
• Versatile: design your own miRNA assays

The QuantiMir Kit is highly sensitive and enables measurement across a wide dynamic range

(A) Starting with total, Trizol-extracted RNA or fractionated small RNA samples you can measure from several micrograms down to picograms of input RNA with excellent accuracy. (B) You can also detect differential miRNA expression across a dynamic range of at least 6 logs.

Material available for download
QuantiMir RT kit brochure
QuantiMir RT kit small RNA quantitation system protocol
Application note: Dissociation analysis of mature and precursor forms of microRNAs using QuantiMir cDNAs
Application note: Quantitate sh/siRNA expression and messenger RNA knockdown levels from a single QuantiMir RT reaction
Cells-to-Cts brief protocol overview

QuantiMir citations