The efficient and simple Quick-cfRNA™ Serum and Plasma Kit enables the purification of high-quality, circulating cell-free RNA from up to 1 ml of plasma, serum and other biological fluids. With no toxic phenol-chlofoform or cumbersome protein precipitation and removal steps, the Quick-cfRNA™ kit enables the purification of RNA suitable for all sensitive downstream analyses including RT-qPCR and next-generation sequencing.
Benefits of using the Quick-cfRNA™ Serum and Plasma Kit
• Simple and reliable protocol
• Ultra-pure, circulating cell-free RNA isolated
• Up to 515x more mRNA recovered compared to competitor kits
• RNA ready for sensitive downstream applications including RT-qPCR and NGS
• No toxic phenol-chloroform
• No cumbersome protein precipitation and removal steps
• Compatible with serum, plasma, saliva, cerebrospinal and amniotic fluid
Quick-cfRNA™ Serum and Plasma Kit is compatible with various biological fluids
Amniotic fluid (AF), cerebrospinal fluid (CSF), or spent HeLa cell culture media (Media) were used to isolate cell-free nucleic acids using Quick-cfDNA/cfRNA™Serum and Plasma Kit. Endogenous cell-free DNA from each sample type was visualised using the Agilent 2200 Tapestation® system.
Quick-cfRNA™ Serum and Plasma Kit gives highest recovery of cell-free miRNA
Cell-free RNA recovery scales proportionally with sample input using the Quick-cfDNA/cfRNA™Serum and Plasma Kit. Cell-free RNA yields from the same plasma donor (61y-F) show linear and efficient recovery of plasma microRNA (hsa-miR-16-5p) when analyzed by RT-qPCR.
Ultra-pure and ready for RT-qPCR
The Quick-cfRNA™ Serum and Plasma Kit results in high purity and recovery of plasma microRNA (hsa-miR-16-5p) based on RT-qPCR analysis.
Material available to download
Quick-cfRNA Serum and Plasma Kit protocol
Note: product availability depends on country - see product detail page.