RNA from serum and plasma: Quick-cfRNA

The efficient and simple Quick-cfRNA™ Serum and Plasma Kit enables the purification of high-quality, circulating cell-free RNA from up to 1 ml of plasma, serum and other biological fluids. With no toxic phenol-chlofoform or cumbersome protein precipitation and removal steps, the Quick-cfRNA™ kit enables the purification of RNA suitable for all sensitive downstream analyses including RT-qPCR and next-generation sequencing.

Benefits of using the Quick-cfRNA™ Serum and Plasma Kit
• Simple and reliable protocol
• Ultra-pure, circulating cell-free RNA isolated
• Up to 515x more mRNA recovered compared to competitor kits
• RNA ready for sensitive downstream applications including RT-qPCR and NGS
• No toxic phenol-chloroform
• No cumbersome protein precipitation and removal steps
• Compatible with serum, plasma, saliva, cerebrospinal and amniotic fluid

Quick-cf Range
Find out more about the Quick-cfDNA™ Serum and Plasma Kit

Quick-cfRNA™ Serum and Plasma Kit is compatible with various biological fluids

Amniotic fluid (AF), cerebrospinal fluid (CSF), or spent HeLa cell culture media (Media) were used to isolate cell-free nucleic acids using Quick-cfDNA/cfRNA™Serum and Plasma Kit.  Endogenous cell-free DNA from each sample type was visualised using the Agilent 2200 Tapestation® system.

Quick-cfRNA™ Serum and Plasma Kit gives highest recovery of cell-free miRNA

Cell-free RNA recovery scales proportionally with sample input using the Quick-cfDNA/cfRNA™Serum and Plasma Kit. Cell-free RNA yields from the same plasma donor (61y-F) show linear and efficient recovery of plasma microRNA (hsa-miR-16-5p) when analyzed by RT-qPCR.

Ultra-pure and ready for RT-qPCR

The Quick-cfRNA™ Serum and Plasma Kit results in high purity and recovery of plasma microRNA (hsa-miR-16-5p) based on RT-qPCR analysis.

Material available to download
Quick-cfRNA Serum and Plasma Kit protocol