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RNA from samples in TRI-Reagent®: Direct-zol

The Direct-zol™ RNA kits from Zymo Research enable the quick, chloroform- and phenol-free purification of high-quality total RNA purification directly from TRI Reagent®, TRIzol®, RNAzol®, QIAzol®, TriPure™, RNA-Bee™ and similar acid-guanidinium-phenol based reagents. These kits can purify up to 100 µg of high-quality total RNA with just seven minutes of preparation time and without phase separation or precipitation.

Benefits of using Direct-zol RNA kits
• Consistent and efficient purification of small and large RNAs
• DNA-free RNA produced
• Seven minute preparation time
• No phenol or chloroform use
• Phenol carryover eliminated
• No phase separation, precipitation or post purification
• Wide sample compatibility
• Compatible with most RNA isolation reagents

Direct-zol RNA kit formats available

Product Catalogue number Binding capacity Format Elution volume Cells Tissue
Direct-zol RNA Microprep R2061 10 µg Spin-column 6 µl ≤1x10 6 ≤5 mg
Direct-zol RNA Miniprep R2051 50 µg Spin-column 25 µl ≤5x10 6 ≤25 mg
Direct-zol RNA Miniprep Plus R2071 100 µg Spin-column 50 µl ≤1x10 7 ≤50 mg
Direct-zol-96 R2055 10 µg 96-well plate 10 µl ≤1x10 6 ≤5 mg
Direct-zol-96 MagBead R2101 10 µg Magnetic beads 50 µl ≤1x10 6 ≤5 mg


Direct-zol workflow vs traditional and competitor methods

  Direct-zol™ Trizol method Supplier A miRNA kit Supplier Q phenol kit
Time per prep 7 min 90+ min 30 min 30 min
No phase separation x x x
NGS-grade x x x
Small RNA recovery x x
DNase I included x x x


Obtain next generation sequencing (NGS) ready RNA with Direct-zol

NGS-Ready DNA with Direct-zol


High quality broad range RNA purified with Direct-zol

Direct-zol high quality RNA

DNA-free RNA purified from human epithelial cells using Direct-zol compared to a DNA containing preparation from Supplier Q (1% agarose/TAE). Small RNAs were effectively recovered with Direct-zol™ while absent in Supplier Q preparations (Agilent Bioanalyzer 2100, Small RNA Chip data shown) (green).

Material available to download
Direct-zol Microprep protocol
Direct-zol Miniprep protocol
Direct-zol Miniprep Plus protocol
Direct-zol -96 RNA kit protocol
Direct-zol -96 Magbead kit protocol
Direct-zol RNA kits datasheet

Free samples
Request a free Direct-zol RNA Miniprep kit sample

Direct-zol RNA citations

TRI Reagent, TRIzol and RNAzol are registered trademarks of Molecular Research Center, Inc. QIAzol is a registered trademark of QIAGEN GmbH. TriPure is a trademark of Roche Diagnostics Operations, Inc. RNA-Bee is a trademark of Tel-Test, Inc.

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Direct-zol RNA Microprep R2060 · Zymo Research
R2060
Zymo Research
50 preparations £195.00
50 preparations
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Direct-zol RNA Microprep (Supplied with 50 ml TRI Reagent) R2061 · Zymo Research
R2061
Zymo Research
50 preparations £274.00
50 preparations
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Direct-zol RNA Microprep R2062 · Zymo Research
R2062
Zymo Research
200 preparations £610.00
200 preparations
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Direct-zol RNA Microprep (Supplied with 200 ml TRI Reagent) R2063 · Zymo Research
R2063
Zymo Research
200 preparations £863.00
200 preparations
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Direct-zol RNA Miniprep R2050 · Zymo Research
R2050
Zymo Research
50 preparations £187.00
50 preparations
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Direct-zol RNA Miniprep (Supplied with 50 ml TRI Reagent) R2051 · Zymo Research
R2051
Zymo Research
50 preparations £271.00
50 preparations
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Direct-zol RNA Miniprep R2052 · Zymo Research
R2052
Zymo Research
200 preparations £587.00
200 preparations
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Direct-zol RNA Miniprep (Supplied with 200 ml TRI Reagent) R2053 · Zymo Research
R2053
Zymo Research
200 preparations £819.00
200 preparations
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Direct-zol RNA Miniprep Plus R2070 · Zymo Research
R2070
Zymo Research
50 preparations £207.00
50 preparations
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Direct-zol RNA Miniprep Plus (Supplied with 50 ml TRI Reagent) R2071 · Zymo Research
R2071
Zymo Research
50 preparations £283.00
50 preparations
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Direct-zol RNA Miniprep Plus R2072 · Zymo Research
R2072
Zymo Research
200 preparations £658.00
200 preparations
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Direct-zol RNA Miniprep Plus (Supplied with 200 ml TRI Reagent) R2073 · Zymo Research
R2073
Zymo Research
200 preparations £910.00
200 preparations
view