www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

Chemiluminescence Western blot substrates: Radiance

The Radiance series of HRP substrates for chemiluminescent Western blots from Azure Biosystems generate long lasting signals and high sensitivity. 

Radiance substrate comparison

Substrate
Description
Benefits
Radiance ECL
An HRP substrate with long lasting signal and sensitivity down to low picograms
• Economical 
• Linear signal for quantitative Westerns
• Use up to 10 times less antibody than other ECL substrates
Radiance Plus
The most sensitive HRP substrate for chemiluminescent Western blotting
• Ultra-high sensitivity for low abundance proteins
• Detect bands not visualised by other substrates
• High signal-to-noise and large dynamic range to quantify low-intensity bands
Radiance Q
Increase the sensitivity and quantitative accuracy of your chemiluminescent Western blots. This substrate is not depleted at high protein loads, resulting in the largest dynamic range of any chemiluminescent substrate.
• Increased sensitivity and quantitative accuracy
• Specifically developed for CCD imaging
• Strong, long-lasting signal with extremely low background
• Ideal for detecting low-abundance proteins
• Higher linear dynamic range for more reliable quantitation

 

Radiance serial dilution

A serial dilution of HeLa lysate was spotted on a membrane and identical dot blots were probed for GAPDH and detected using the Radiance Substrate indicated.

Radiance substrates are better optimised for quantitation than alternatives

Chemiluminescence Comparison With Radiance Substrates

Radiance ECL shows significantly less signal decay than other substrates

The blots pictured to the right were imaged  at regular intervals up to sixty minutes, and  the signal of the second spot is plotted above. Radiance ECL shows significantly less signal  decay than other substrates

Radiance ECL signal linearity

Radiance ECL signal linearity

A 1:1 serial dilution of HeLa lysate was spotted on a membrane and identical dot blots were probed for GAPDH and detected using the substrate indicated.The linearity of the Radiance ECL signal is shown on the bottom.

Free samples
Request a free sample

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Radiance Q AC2101 · Azure Biosystems
AC2101
Azure Biosystems
150 ml £250.00
150 ml
view
Radiance ECL AC2204 · Azure Biosystems
AC2204
Azure Biosystems
500 ml £265.00
500 ml
view
Radiance Plus AC2103 · Azure Biosystems
AC2103
Azure Biosystems
150 ml £290.00
150 ml
view