Chemiluminescence Western blot substrates: Radiance

The Radiance series of HRP substrates for chemiluminescent Western blots from Azure Biosystems generate long lasting signals and high sensitivity.

Radiance substrate comparison

Substrate	Description	Benefits
Radiance ECL	An HRP substrate with long lasting signal and sensitivity down to low picograms	• Economical • Linear signal for quantitative Westerns • Use up to 10 times less antibody than other ECL substrates
Radiance Plus	The most sensitive HRP substrate for chemiluminescent Western blotting	• Ultra-high sensitivity for low abundance proteins • Detect bands not visualised by other substrates • High signal-to-noise and large dynamic range to quantify low-intensity bands
Radiance Q	Increase the sensitivity and quantitative accuracy of your chemiluminescent Western blots. This substrate is not depleted at high protein loads, resulting in the largest dynamic range of any chemiluminescent substrate.	• Increased sensitivity and quantitative accuracy • Specifically developed for CCD imaging • Strong, long-lasting signal with extremely low background • Ideal for detecting low-abundance proteins • Higher linear dynamic range for more reliable quantitation

Radiance serial dilution

A serial dilution of HeLa lysate was spotted on a membrane and identical dot blots were probed for GAPDH and detected using the Radiance Substrate indicated.

Radiance substrates are better optimised for quantitation than alternatives

Chemiluminescence Comparison With Radiance Substrates

Radiance ECL shows significantly less signal decay than other substrates

The blots pictured to the right were imaged at regular intervals up to sixty minutes, and the signal of the second spot is plotted above. Radiance ECL shows significantly less signal decay than other substrates

Radiance ECL signal linearity

A 1:1 serial dilution of HeLa lysate was spotted on a membrane and identical dot blots were probed for GAPDH and detected using the substrate indicated.The linearity of the Radiance ECL signal is shown on the bottom.

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Products

Note: product availability depends on country - see product detail page.

Details	Cat number & supplier	Size	Price
Radiance Q AC2101 · Azure Biosystems	AC2101 Azure Biosystems	150 ml	£305.00 150 ml view
Radiance ECL AC2204 · Azure Biosystems	AC2204 Azure Biosystems	500 ml	£343.00 500 ml view
Radiance Plus AC2103 · Azure Biosystems	AC2103 Azure Biosystems	150 ml	£361.00 150 ml view

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