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Promoter cloning and expression lentivectors

The promoter cloning and expression lentivectors from System Biosciences (SBI) are effective in delivery and stable expression of any cDNA in a wide range of mammalian cells. With a wide selection of promoter options available including CMV (Cytomegalovirus), MSCV (Murine Stem Cell Virus), UbC (Ubiquitin C), PGK (Phosphoglycerate Kinase) and EF1 (Elongation Factor 1α), deliver your cDNA construct of interest to both dividing and non-dividing cells.

Benefits of using these lentivectors
• Multiple promoter options
• Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
• Single promoter, dual promoter and bidirectional promoter formats
• High expression in most hematopoietic, embryonic and stem cells via the MSCV CpG-deficient promoter

Promotor Expression level Application
CMV High Commonly used in most cell lines (HeLa, HEK293, HT1080 etc.)
MSCV High Hematopoietic and stem cells
EF1α Medium Most cell types including primary cells and stem cells
PGK Medium Most cell types including primary cells and stem cells
UbC Low Most cell types including primary cells and stem cells


Effective transduction with SBI’s bidirectional promoter lentivectors

Sample data showing effective transduction with SBI’s Bidirectional Promoter Lentivectors

HEK293 cells were transduced with pCDH-EF1α-MCS-(PGK-Puro) (Cat.# CDH810A-1, upper left panel), pCDH-EF1α-MCS-(PGK-GFP-T2A-Puro) (Cat.# CDH813A-1, upper right panel), pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) (Cat.# CDH822A-1, bottom left panel), pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) (Cat.# CDH823A-1, bottom right panel). After four days, cells were imaged—the high number of cells present after puromycin selection (upper left panel) and cells showing GFP or RFP signal (all other panels) demonstrates the effectiveness of this lentivector family.

Material available for download
pCDH cDNA cloning and expression lentivectors manual
pCDF cDNA cloning and expression lentivectors manual

Promoter cloning and expression lentivectors citations

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
pCDH-EF1-MCS-(PGK-Puro) Cloning and Expression Vector CD810A-1 · System Biosciences
CD810A-1
System Biosciences
10 µg £539.00
10 µg
view
pCDH-EF1-MCS-(PGK-GFP) Cloning and Expression Vector CD811A-1 · System Biosciences
CD811A-1
System Biosciences
10 µg £539.00
10 µg
view
pCDH-EF1-MCS-(PGK-GFP-T2A-Puro) Cloning and Expression Vector CD813A-1 · System Biosciences
CD813A-1
System Biosciences
10 µg £539.00
10 µg
view
pCDH-EF1-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Vector CD822A-1 · System Biosciences
CD822A-1
System Biosciences
10 µg £539.00
10 µg
view
pCDH-EF1-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Vector CD823A-1 · System Biosciences
CD823A-1
System Biosciences
10 µg £539.00
10 µg
view
pCDF1-MCS1 cDNA Cloning and Expression Vector CD100A-1 · System Biosciences
CD100A-1
System Biosciences
10 µg £450.00
10 µg
view
pCDF1-MCS2-EF1-Puro cDNA Cloning and Expression Lentivector CD110B-1 · System Biosciences
CD110B-1
System Biosciences
10 µg £532.00
10 µg
view
pCDF1-MCS2-EF1-copGFP cDNA Cloning and Expression Vector CD111B-1 · System Biosciences
CD111B-1
System Biosciences
10 µg £532.00
10 µg
view
pCDH-CMV-MCS cDNA Cloning and Expression Vector CD500B-1 · System Biosciences
CD500B-1
System Biosciences
10 µg £450.00
10 µg
view
pCDH-CMV-MCS2 cDNA Cloning and Expression Vector CD501A-1 · System Biosciences
CD501A-1
System Biosciences
10 µg £450.00
10 µg
view
pCDH-EF1-MCS cDNA Cloning and Expression Vector CD502A-1 · System Biosciences
CD502A-1
System Biosciences
10 µg £450.00
10 µg
view
pCDH-CMV-MCS-EF1-copGFP cDNA Cloning and Expression Vector CD511B-1 · System Biosciences
CD511B-1
System Biosciences
10 µg £532.00
10 µg
view
pCDH-CMV-MCS-EF1-RFP cDNA Cloning and Expression Vector CD512B-1 · System Biosciences
CD512B-1
System Biosciences
10 µg £532.00
10 µg
view
pCDH-CMV-MCS-EF1-GreenPuro cDNA Cloning and Expression Vector CD513B-1 · System Biosciences
CD513B-1
System Biosciences
10 µg £532.00
10 µg
view
pCDH-CMV-MCS-EF1-Neo cDNA Cloning and Expression Vector CD514B-1 · System Biosciences
CD514B-1
System Biosciences
10 µg £532.00
10 µg
view
pCDH-CMV-MCS-EF1-Hygro cDNA Cloning and Expression Vector CD515B-1 · System Biosciences
CD515B-1
System Biosciences
10 µg £532.00
10 µg
view
pCDH-CMV-MCS-EF1-RFP+Puro cDNA Cloning and Expression Vector CD516B-2 · System Biosciences
CD516B-2
System Biosciences
10 µg £532.00
10 µg
view
pCDH-MCS-T2A-Puro-MSCV cDNA Cloning and Expression Vector CD522A-1 · System Biosciences
CD522A-1
System Biosciences
10 µg £548.00
10 µg
view
pCDH-MCS-T2A-copGFP-MSCV Cloning and Expression Vector CD523A-1 · System Biosciences
CD523A-1
System Biosciences
10 µg £548.00
10 µg
view
pCDH-CMV-MCS-t2A-copGFP cDNA Cloning and Expression Vector CD524A-1 · System Biosciences
CD524A-1
System Biosciences
10 µg £548.00
10 µg
view
pCDH-EF1-MCS*-T2A-GFP CD526A-1 · System Biosciences
CD526A-1
System Biosciences
10 µg £548.00
10 µg
view
pCDH-EF1-MCS-T2A-Puro cDNA Cloning and Expression Vector CD527A-1 · System Biosciences
CD527A-1
System Biosciences
10 µg £548.00
10 µg
view
pCDH-EF1-MCS-IRES-GFP cDNA Cloning and Expression Vector CD530A-2 · System Biosciences
CD530A-2
System Biosciences
10 µg £548.00
10 µg
view
pCDH-EF1-MCS-IRES-RFP cDNA Cloning and Expression Vector CD531A-2 · System Biosciences
CD531A-2
System Biosciences
10 µg £548.00
10 µg
view
pCDH-EF1-MCS-IRES-Puro cDNA Cloning and Expression Vector CD532A-2 · System Biosciences
CD532A-2
System Biosciences
10 µg £548.00
10 µg
view