Promoter cloning and expression lentivectors

The promoter cloning and expression lentivectors from System Biosciences (SBI) are effective in delivery and stable expression of any cDNA in a wide range of mammalian cells. With a wide selection of promoter options available including CMV (Cytomegalovirus), MSCV (Murine Stem Cell Virus), UbC (Ubiquitin C), PGK (Phosphoglycerate Kinase) and EF1 (Elongation Factor 1α), deliver your cDNA construct of interest to both dividing and non-dividing cells.

Benefits of using these lentivectors
• Multiple promoter options
• Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
• Single promoter, dual promoter and bidirectional promoter formats
• High expression in most hematopoietic, embryonic and stem cells via the MSCV CpG-deficient promoter

Effective transduction with SBI’s bidirectional promoter lentivectors

HEK293 cells were transduced with pCDH-EF1α-MCS-(PGK-Puro) (Cat.# CDH810A-1, upper left panel), pCDH-EF1α-MCS-(PGK-GFP-T2A-Puro) (Cat.# CDH813A-1, upper right panel), pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) (Cat.# CDH822A-1, bottom left panel), pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) (Cat.# CDH823A-1, bottom right panel). After four days, cells were imaged—the high number of cells present after puromycin selection (upper left panel) and cells showing GFP or RFP signal (all other panels) demonstrates the effectiveness of this lentivector family.

Material available for download
pCDH cDNA cloning and expression lentivectors manual
pCDF cDNA cloning and expression lentivectors manual

Promoter cloning and expression lentivectors citations