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Precast Bis-Tris PAGE gels

This range of precast gels for protein gel electrophoresis from GenScript offer even, sharp bands with guaranteed lot-to-lot consistency. The Bis-Tris gels are cast and run at a neutral pH to minimise polyacrylamide hydrolysis, increase gel stability and minimise protein modication compared with Tris-glycine gels. Compatible with all popular mini-gel tanks and provided with up to 12 months' shelf life, the SurePAGE gels offer a higher resolution and cost-effective option for protein gel electrophoresis.

Benefits of using GenScript precast gels
• Large well volume – up to 80 µl
• High resolution for even, sharp bands
• Guaranteed lot-to-lot consistency
• Long shelf life – up to 12 months at 2-8°C
• Compatible with all popular mini-gel tanks
• Gel stability increased
• Polyacrylamide hydrolysis minimised
• Reduced protein modification compared to Tris-glycine gels
• Cost effective
• Free samples available

The range includes:

  • SurePAGE™ gels offering higher resolution and reproducibility
  • ExpressPlus™ gels which are great quality and cost-effective gels

SurePAGE gels offer superior band resolution compared to competitors and homemade Tris-Glycine gels

SurePAGE gels offer superior band resolution compared to competitors and homemade Tris-Glycine gels

Lane 1 and 5: protein marker (MM1397), 5 µl. Lane 2, 3, 4, 6, 7, 8 and 9: E. coli 10ul cell lysate

Material available for download
SurePAGE SurePAGE precast gels brochure
ExpressPlus PAGE gels technical manual
SurePAGE gels technical manual
ExpressPlus PAGE gels quick guide
SurePAGE gels quick guide

Applications
• Protein electrophoresis
• Western blotting

Steps to check prior to ordering:

Check the compatibility of your gel tank

Compatible gel tanks

*please reverse the gasket, see manual

Bio-Rad and PROTEAN are trademarks of Bio-Rad Laboratories

Choose the gel that best separates your protein of interest based on the migration chart below:

Step 2

 

Choose the appropriate well number based on the number of samples and sample volume

Gel series Well number Recommended max. loading volume per well Well size Recommended amount of protein per well
10 x 8 gels 10 60 µl 80 µl 60 µg
12 45 µl 60 µl 50 µg
15 30 µl 40 µl 40 µg

Choose LDS based sample loading buffer

For best result, GenScript recommend using 4XLDS sample buffer (M00676) as the sample loading buffer.

An alternative sample buffer is 5x SDS sample buffer (MB01015). The figure below shows that compared to SDS sample buffer, LDS sample buffer enables better separation of the protein samples.

Choose buffer

 

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Tris-MOPS-SDS Running Buffer Powder M00138 · GenScript Biotech
M00138
GenScript Biotech
5 pack £28.00
5 pack
view
Transfer Buffer Powder M00139 · GenScript Biotech
M00139
GenScript Biotech
10 pack £64.00
10 pack
view
Broad Multi Color Pre-Stained Protein Standard M00624-1250 · GenScript Biotech
M00624-1250
GenScript Biotech
5 x 250 µl £306.00
5 x 250 µl
view
Broad Multi Color Pre-Stained Protein Standard M00624-250 · GenScript Biotech
M00624-250
GenScript Biotech
250 µl £77.00
250 µl
view
SurePAGE™, Bis-Tris, 10 x 8, 4-12%, 10 wells M00652 · GenScript Biotech
M00652
GenScript Biotech
10 pack £93.00
10 pack
view
SurePAGE™, Bis-Tris, 10 x 8, 4-12%, 12 wells M00653 · GenScript Biotech
M00653
GenScript Biotech
10 pack £93.00
10 pack
view
SurePAGE™, Bis-Tris, 10 x 8, 4-12%, 15 wells M00654 · GenScript Biotech
M00654
GenScript Biotech
10 pack £93.00
10 pack
view
SurePAGE™, Bis-Tris, 10 x 8, 4-20%, 10 wells M00655 · GenScript Biotech
M00655
GenScript Biotech
10 pack £93.00
10 pack
view
SurePAGE™, Bis-Tris, 10 x 8, 4-20%, 12 wells M00656 · GenScript Biotech
M00656
GenScript Biotech
10 pack £93.00
10 pack
view
SurePAGE™, Bis-Tris, 10 x 8, 4-20%, 15 wells M00657 · GenScript Biotech
M00657
GenScript Biotech
10 pack £93.00
10 pack
view
SurePAGE™, Bis-Tris, 10 x 8, 8-16%, 10 wells M00658 · GenScript Biotech
M00658
GenScript Biotech
10 pack £93.00
10 pack
view
SurePAGE™, Bis-Tris, 10 x 8, 8-16%, 12 wells M00659 · GenScript Biotech
M00659
GenScript Biotech
10 pack £93.00
10 pack
view
SurePAGE™, Bis-Tris, 10 x 8, 8-16%, 15 wells M00660 · GenScript Biotech
M00660
GenScript Biotech
10 pack £93.00
10 pack
view
SurePAGE™, Bis-Tris, 10 x 8, 8%, 10 wells M00661 · GenScript Biotech
M00661
GenScript Biotech
10 pack £93.00
10 pack
view
SurePAGE™, Bis-Tris, 10 x 8, 8%, 12 wells M00662 · GenScript Biotech
M00662
GenScript Biotech
10 pack £93.00
10 pack
view
SurePAGE™, Bis-Tris, 10 x 8, 8%, 15 wells M00663 · GenScript Biotech
M00663
GenScript Biotech
10 pack £93.00
10 pack
view
SurePAGE™, Bis-Tris, 10 x 8, 10%, 10 wells M00664 · GenScript Biotech
M00664
GenScript Biotech
10 pack £93.00
10 pack
view
SurePAGE™, Bis-Tris, 10 x 8, 10%, 12 wells M00665 · GenScript Biotech
M00665
GenScript Biotech
10 pack £93.00
10 pack
view
SurePAGE™, Bis-Tris, 10 x 8, 10%, 15 wells M00666 · GenScript Biotech
M00666
GenScript Biotech
10 pack £93.00
10 pack
view
SurePAGE™, Bis-Tris, 10 x 8, 12%, 10 wells M00667 · GenScript Biotech
M00667
GenScript Biotech
10 pack £93.00
10 pack
view
SurePAGE™, Bis-Tris, 10 x 8, 12%, 12 wells M00668 · GenScript Biotech
M00668
GenScript Biotech
10 pack £93.00
10 pack
view
SurePAGE™, Bis-Tris, 10 x 8, 12%, 15 wells M00669 · GenScript Biotech
M00669
GenScript Biotech
10 pack £93.00
10 pack
view
4X LDS Sample Buffer M00676-10 · GenScript Biotech
M00676-10
GenScript Biotech
10 ml £11.00
10 ml
view
4X LDS Sample Buffer M00676-250 · GenScript Biotech
M00676-250
GenScript Biotech
250 ml £99.00
250 ml
view
MES SDS Running Buffer Powder M00677 · GenScript Biotech
M00677
GenScript Biotech
5 pieces £28.00
5 pieces
view