Low input whole genome bisulphite sequencing library prep: Pico Methyl-Seq

The Pico Methyl-Seq™ Library Prep Kit from Zymo Research enables all-inclusive bisulphite conversion and whole genome bisulphite sequencing (WGBS) library preparation with a streamlined, ligation- and gel-free method. Due to the three rounds of amplification included in the workflow, this kit can accommodate inputs as low as 10 pg DNA (including FFPE-derived), making it ideal for methylation analysis of valuable, ultra-low input DNA samples.

Benefits of using the Pico Methyl-Seq Library Prep Kit

• All-inclusive kit for bisulphite conversion and WGBS library prep
• Simple, ligation- and gel-free workflow
• Ultra-low input ≥ 10 pg DNA
• Compatible with FFPE samples
• Enables whole-genome analysis of 5-methylcytosine

Pico Methyl-Seq offers a simple protocol for WGBS library preparation

Validation of Pico Methyl-Seq libraries. Agilent 2200 TapeStation® D1K gel of libraries prepared (from B1-G1) using 10 pg, 20 pg, 100 pg, 1 ng, 10 ng, and 100 ng, respectively.

Applications of Pico Methyl-Seq

• WGBS Library Preparation
• Epigenetic analysis of DNA methylation

Material available for download

Pico Methyl-Seq Library Prep Kit protocol

TapeStation® is a registered trademark of Agilent Technologies, Inc.