Nucleic acid labelling kits: Label IT

The Label IT® technology from Mirus Bio is a non-enzymatic, chemical labelling method for direct covalent attachment of either fluorescent or non-fluorescent labels to any nucleic acid. Using a simple, one-step chemical reaction, these kits are optimised for specialised applications including nucleic acid visualisation both in vitro and in vivo

How the Label IT Nucleic Acid Labelling Kits work

Label IT technology

Label IT Nucleic Acid Labelling Kits - Label IT chemical labelling reagents are composed of three regions: the label (fluorophore or hapten) (green), the linker (yellow) which facilitates electrostatic interactions with nucleic acids and the reactive alkylating group (blue) that covalently attaches the Label IT® reagent to any reactive heteroatom within the nucleic acids. 

Benefits of using the Label IT kits
• Label any DNA or RNA template
• Single-step chemical method
• Adjustable labelling density
• Covalent mechanism does not impact hybridisation performance
• Label and deliver plasmid DNA for intracellular tracking experiments
• Tracking of functional fluorescently labelled siRNA 

Successful delivery and functional knockdown using labelled siRNA

Successful delivery and functional knockdown using labelled siRNA

(A) HeLa cells in 12-well plates were transfected at 70% confluence with TransIT-TKO® Transfection Reagent (3 μl/well) and Label IT siRNA Tracker Fluorescein-labelled siRNA duplexes (GREEN, 50 nM final concentration in the well). The cells were incubated 24 hours post-transfection then fixed and counterstained with TO-PRO®-3 (nuclei, BLUE) and Alexa Fluor® 546 Phalloidin (actin, RED). Confocal images were acquired on a Zeiss LSM 510 Confocal Microscope. (B) TransIT-TKO Transfection Reagent was used to transfect anti-firefly luciferase siRNA into CHO-Luc cells stably expressing firefly luciferase. The siRNA was either unlabeled or labeled with Label IT siRNA Tracker Cy®3, Cy®5, Fluorescein, or CX-Rhodamine Reagents. Bars indicate the percent firefly luciferase expression 24 hours after delivery of 5 nM anti-firefly luciferase siRNA.

Material available for download
Label IT Nucleic Acid Labelling Kits protocol
Label IT Nucleic Acid Labelling Kits quick reference protocol
Label IT Nucleic Acid Labelling Kits SDS
Label IT Amine Full Labelling protocol
Label IT siRNA Tracker™ Kit Full Labelling protocol
Label IT Tracker Kit Full Labelling protocol
Technical report: High efficiency siRNA delivery in vitro
Intracellular localisation and expression of labelled plasmid DNA using Label IT Tracker Nucleic Acid Labelling Kits
Nucleic acid labelling and delivery tools for neurobiology

Contact a specialist
To discuss your requirements, please contact our Mirus specialists.

TO-PRO and Alexa Fluor are registered trademarks of Thermo Fisher Scientific. Cy is a registered trademark of GE Healthcare UK Limited.

Products

Note: product availability depends on country - see product detail page.

Details Cat number & supplier Size Price
Label IT® Nucleic Acid Labeling Kit, MFP488 MIR 7125 · Mirus
MIR 7125
Mirus
Labels 25 µg nucleic acid £289.00
Labels 25 µg nucleic acid
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Label IT® Nucleic Acid Labeling Kit, MFP488 MIR 7100 · Mirus
MIR 7100
Mirus
Labels 100 µg nucleic acid £722.00
Labels 100 µg nucleic acid
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Label IT® Nucleic Acid Labeling Kit, Biotin MIR 3400 · Mirus
MIR 3400
Mirus
Labels 100 µg nucleic acid £722.00
Labels 100 µg nucleic acid
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Label IT® Nucleic Acid Labeling Kit, Biotin MIR 3425 · Mirus
MIR 3425
Mirus
Labels 25 µg nucleic acid £289.00
Labels 25 µg nucleic acid
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Label IT® Nucleic Acid Labeling Kit, CX-Rhodamine MIR 3100 · Mirus
MIR 3100
Mirus
Labels 100 µg nucleic acid £722.00
Labels 100 µg nucleic acid
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Label IT® Nucleic Acid Labeling Kit, CX-Rhodamine MIR 3125 · Mirus
MIR 3125
Mirus
Labels 25 µg nucleic acid £289.00
Labels 25 µg nucleic acid
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Label IT® Nucleic Acid Labeling Kit, Cy®3 MIR 3600 · Mirus
MIR 3600
Mirus
Labels 100 µg nucleic acid £852.00
Labels 100 µg nucleic acid
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Label IT® Nucleic Acid Labeling Kit, Cy®3 MIR 3625 · Mirus
MIR 3625
Mirus
Labels 25 µg nucleic acid £343.00
Labels 25 µg nucleic acid
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Label IT® Nucleic Acid Labeling Kit, Cy®5 MIR 3700 · Mirus
MIR 3700
Mirus
Labels 100 µg nucleic acid £852.00
Labels 100 µg nucleic acid
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Label IT® Nucleic Acid Labeling Kit, Cy®5 MIR 3725 · Mirus
MIR 3725
Mirus
Labels 25 µg nucleic acid £343.00
Labels 25 µg nucleic acid
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Label IT® Nucleic Acid Labeling Kit, DNP MIR 3800 · Mirus
MIR 3800
Mirus
Labels 100 µg nucleic acid £722.00
Labels 100 µg nucleic acid
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Label IT® Nucleic Acid Labeling Kit, DNP MIR 3825 · Mirus
MIR 3825
Mirus
Labels 25 µg nucleic acid £289.00
Labels 25 µg nucleic acid
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Label IT® Nucleic Acid Labeling Kit, Fluorescein MIR 3200 · Mirus
MIR 3200
Mirus
Labels 100 µg nucleic acid £722.00
Labels 100 µg nucleic acid
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Label IT® Nucleic Acid Labeling Kit, Fluorescein MIR 3225 · Mirus
MIR 3225
Mirus
Labels 25 µg nucleic acid £289.00
Labels 25 µg nucleic acid
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Label IT® Nucleic Acid Labeling Kit, TM-Rhodamine MIR 4100 · Mirus
MIR 4100
Mirus
Labels 100 µg nucleic acid £722.00
Labels 100 µg nucleic acid
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Label IT® Nucleic Acid Labeling Kit, TM-Rhodamine MIR 4125 · Mirus
MIR 4125
Mirus
Labels 25 µg nucleic acid £289.00
Labels 25 µg nucleic acid
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Label IT® Nucleic Acid Modifying Kit, Amine MIR 3900 · Mirus
MIR 3900
Mirus
Sufficient reagent for modifying 100 µg of nucleic acid £852.00
Sufficient reagent for modifying 100 µg of nucleic acid
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Label IT® Nucleic Acid Modifying Kit, Amine MIR 3925 · Mirus
MIR 3925
Mirus
Sufficient reagent for modifying 25 µg of nucleic acid £343.00
Sufficient reagent for modifying 25 µg of nucleic acid
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Label IT® Plasmid Delivery Control, Cy®3 MIR 7904 · Mirus
MIR 7904
Mirus
10 µg £196.00
10 µg
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Label IT® Plasmid Delivery Control, Cy®3 MIR 7905 · Mirus
MIR 7905
Mirus
100 µg £954.00
100 µg
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Label IT® Plasmid Delivery Control, Fluorescein MIR 7906 · Mirus
MIR 7906
Mirus
10 µg £151.00
10 µg
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Label IT® RNAi Delivery Control, Cy®3 MIR 7900 · Mirus
MIR 7900
Mirus
10 µg (0.75 nmol) £196.00
10 µg (0.75 nmol)
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Label IT® RNAi Delivery Control, Cy®3 MIR 7901 · Mirus
MIR 7901
Mirus
100 µg (7.5 nmol) £954.00
100 µg (7.5 nmol)
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Label IT® siRNA Tracker Intracellular Localization Kit, Biotin Labeling MIR 7217 · Mirus
MIR 7217
Mirus
Labels 50 µg siRNA £502.00
Labels 50 µg siRNA
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Label IT® siRNA Tracker Intracellular Localization Kit, CX-Rhodamine MIR 7214 · Mirus
MIR 7214
Mirus
Labels 50 µg siRNA £502.00
Labels 50 µg siRNA
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