Human methylated and nonmethylated DNA set

Purified methylated and nonmethylated human DNA can be used as positive and negative controls in methylation detection applications. Complete with a set of control primers, the DNA control sets from Zymo Research are ideal for use in conjunction with their range of EZ DNA Methylation™ kits.

Human HCT116 DKO methylated DNA
The Human HCT116 DKO methylated DNA is purified HCT116 DKO DNA that has been enzymatically methylated at all cytosine positions comprising CG dinucleotides by M.SssI methyltransferase2 and is ideal for use as a positive control for DNA methylation analysis.

Human HCT116 DKO nonmethylated DNA
The Human HCT116 DKO nonmethylated DNA is purified from cells containing genetic knockouts of both DNA methyltransferases DNMT1 (-/-) and DNMT3b (-/-)1. DNA derived from HCT116 DKO cells has low levels of DNA methylation and is ideal for use as a control for DNA methylation analysis.

An assay for complete methylation by M.SssI methytransferase. Digestion of nonmethylated and methylated HCT116 DKO DNA with restriction enzymes MspI and HpaII. MspI digests both nonmethylated and methylated DNA. HpaII is sensitive to CpG methylation.

EZ DNA Methylation Kits
Find out more about Zymo's range of high-efficiency DNA bisulphite conversion kits

Material available for download
Human Methylated and Nonmethylated DNA Set data sheet
Human methylated and nonmethylated DNA set protocol